Postdoctoral Fellow - PGC And Gene Modification Studies In Falcons

Job Description

We are seeking a highly motivated and skilled Research Scientist to join the Khalifa Center for Genetic Engineering and Biotechnology (KCGEB) for a groundbreaking research project focused on genetic manipulation of primordial germ cells (PGCs) to modify plumage color in falcons. This project will use advanced CRISPR/Cas9 gene-editing technology to alter the melanin related genes, aiming to produce falcons with white or light-colored plumage. The project also involves building the capacity for PGC culture, gene editing, and breeding techniques at the Biology Department of UAE University. This is a multidisciplinary project with a strong focus on genome editing, embryology, and avian biology, aimed at developing novel methods for breeding falcons with desired phenotypic traits. Key Responsibilities: 1- Perform genome editing using the CRISPR/Cas9 system to target the MC1R gene in avian primordial germ cells (PGCs) to alter plumage color. 2- Develop protocols for the isolation, culture, and transfection of PGCs from both chicken and falcon embryos. 3- Conduct phenotypic and genetic screening of transgenic falcons to assess the success of gene modifications. 4- Optimize methods for PGC cryopreservation and perform long-term storage of cultured cells. 5- Perform laboratory tasks including DNA/RNA isolation, plasmid design, transfections, PCR, and gene expression analysis using techniques like 6- Western blotting and flow cytometry. 7- Collaborate with interdisciplinary teams of geneticists, biologists, and veterinarians to ensure the successful implementation of the project goals. 8- Provide training to students and other researchers on the methodologies being developed. 9- Ensure compliance with all biosafety and ethical protocols, including obtaining animal ethics approvals. 10- Prepare regular reports and scientific publications related to the research outcomes.

Minimum Qualification

• PhD in Molecular Biology, Genetics, Biotechnology, or a related field.
• Previous experience working with avian species, particularly chickens and/or falcons, in a research setting.
• Knowledge of melanin production and pigmentation pathways, particularly relating to the MC1R gene.
• Experience in cryopreservation techniques for germ cells or stem cells.
• Familiarity with FACS and flow cytometry for cell sorting and analysis.
  
  

Preferred Qualification

• PhD in Molecular Biology, Genetics, Biotechnology, or a related field.
• Previous experience working with avian species, particularly chickens and/or falcons, in a research setting.
• Knowledge of melanin production and pigmentation pathways, particularly relating to the MC1R gene.
• Experience in cryopreservation techniques for germ cells or stem cells.
• Familiarity with FACS and flow cytometry for cell sorting and analysis.
  
  

Expected Skills

1- PhD in Molecular Biology, Genetics, Biotechnology, or a related field. 2- Proven experience with CRISPR/Cas9 genome editing technology. 3- Hands-on experience with PGC isolation, cell culture, and transfection techniques, preferably in avian or related species. 4- Strong knowledge of molecular biology techniques including DNA/RNA extraction, PCR, gene expression analysis, and Western blotting. 5- Familiarity with embryology and PGC manipulation in birds or similar models (e.g., chicken or other avian species). 6- Excellent problem-solving skills with the ability to troubleshoot experimental issues. 7- Strong communication and scientific writing skills, with experience in preparing reports and research publications. 8- Ability to work both independently and in a collaborative team environment.

Close Date Kindly apply before the closing date.

Open until filled

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